Blood collection and administration of fluids in mouse, حصريا بالصور

السلام عليكــم ورحمـة الله وبركاتــة ،،



Administration of Fluids and Drugs (Overview):
When drugs, vaccines, injectable anesthetics or other agents are to be administered, one of several different routes may be selected. The route is governed by the nature of the agent being administered, the animal, and the purpose of the administration, among other factors.



Gastro-Intestinal Tract:
Oral: Substances may be administered orally by addition to the food or the drinking water. The amount of drug consumed by an animal will vary between each individual. If the drug imparts an unpleasant taste, affecting palatability, the drug will be consumed in decreased amounts. Also, environmental conditions such as the ambient temperature will also affect both water and food consumption. The general rule of thumb is that 15 grams of food and 15 mls of water will be consumed daily per 100 grams of body weight.


Gavage: If it is necessary to administer exact amounts of a substance gastric feeding needles should be used.


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Parenteral Administration: General Considerations

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Intravenous:



Equipment: 27-30 g needle, 1 ml tuberculin syringe, mouse holder, warming lamp.


Volume: The volume injected IV into an adult mouse should not exceed 0.2 ml.



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Intraperitoneal:
Equipment: Syringe and 23 to 27 g, 1/2 to 1-inch needle, preferably with a short bevel.


Volume: The volume injected IP into an adult mouse should not exceed 2 ml.


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Subcutaneous:
Equipment: Syringe and 25 to 27 g, 1/2 to 3/4-inch needle.


Volume: The volume injected SQ into an adult mouse should not exceed 2-3 ml.



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Blood Collection





Site Preparation:
The area of injection or incision should be cleaned with alcohol. Some procedures will require sedation or anesthesia; others may be carried out without anesthesia provided suitable restraint is used. In order to better visualize veins dilation can be accomplished by immersing the tail in warm water for 5 to 10 seconds or by warming the animal with a low-wattage light bulb for 5 to 15 minutes prior to venipuncture. This also aids by providing additional light


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Tail vein venipuncture in mice:
Equipment: scalpel blade, 25 to 30 gauge needle


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Toe clipping or tail clipping to obtain blood samples: Clipping toes is an unacceptable method of blood collection. Tail clipping is not a preferable method for blood collection.



Cardiac puncture:
Equipment: 0.90 to 0.50 mm needle



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